GeneTex
United States (US)

RanGAP1 antibody [N1N3]

GTX114700_40219_20160825_WB_M_18121410_422.jpg
GTX114700_40219_20160825_WB_18121410_764.jpg
GTX114700_40219_20160825_WB_R_18121410_677.jpg

Cat No. GTX114700

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RanGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ran Gtpase Activating Protein 1,Fug1,Rangap,Sd,Rangap1

Cellular Localization

Cytoplasm , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side , Kinetochore

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114700_40219_20160825_WB_M_18121410_422.jpg

GTX114700 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

GTX114700_40219_20160825_WB_18121410_764.jpg

GTX114700 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:5000.
The observed M.W. is based on the publication: PMID: 18706886

GTX114700_40219_20160825_WB_R_18121410_677.jpg

GTX114700 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169