GeneTex
  • Country / Location Selection

United States (US)

RanGAP1 antibody [N1N3]

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700
Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700
Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

Cat No. GTX114700

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RanGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620615

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ran GTPase activating protein 1 , Fug1 , RANGAP , SD

Cellular Localization

Cytoplasm , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side , Kinetochore

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:5000.
The observed M.W. is based on the publication: PMID: 18706886

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

REFERENCE

There are currently no references for RanGAP1 antibody [N1N3] (GTX114700). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RanGAP1 antibody [N1N3] (GTX114700). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169