GeneTex
United States (US)

Rap1 antibody [1D2-1C64]

GTX31146_761_ICC-IF_19011519_240.jpg
GTX31146_1452_IP_19011519_973.jpg
GTX31146_1345_IHC-P_19011519_877.jpg
GTX31146_1854_WB_19011519_493.jpg
GTX31146_762_ICC-IF_19011519_970.jpg

Cat No. GTX31146

Host Mouse
Clonality Monoclonal
Clone Name 1D2-1C64
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:10-1:200
IHC-P 1:100-1:1000
IP 2 μg
Not tested in other applications.

Calculated MW

21 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 30% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human Rap1

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C21KG,G-22K,KREV-1,KREV1,RAP1,SMGP21

Cellular Localization

Cell junction,Cell membrane,Cytoplasm,Early endosome,Perinuclear region

Background

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
DATA IMAGES
GTX31146_761_ICC-IF_19011519_240.jpg

GTX31146 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31146 Rap1 antibody [1D2-1C64].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

GTX31146_1452_IP_19011519_973.jpg

GTX31146 IP Image

IP analysis of NIH3T3 cell lysates using GTX31146 Rap1 antibody [1D2-1C64].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:500

GTX31146_1345_IHC-P_19011519_877.jpg

GTX31146 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31146 Rap1 antibody [1D2-1C64].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

GTX31146_1854_WB_19011519_493.jpg

GTX31146 WB Image

WB analysis of 25 µg of various whole cell lysates per well using GTX31146 Rap1 antibody [1D2-1C64].
Dilution : 1:500

GTX31146_762_ICC-IF_19011519_970.jpg

GTX31146 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX31146 Rap1 antibody [1D2-1C64]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

Package List Price ($)
$ 319