APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:10-1:200 |
| 1:100-1:1000 |
| 2 μg |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 30% Glycerol, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human Rap1
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
RAP1A, member of RAS oncogene family , C21KG , G-22K , KREV-1 , KREV1 , RAP1 , SMGP21
Cellular Localization
Cell membrane,Cytoplasm,Cell junction
Background
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
![Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146](/templates/default/js/lightbox/images/loading.gif "Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146")
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GTX31146 IHC-P Image
IHC-P analysis of human kidney tissue using GTX31146 Rap1 antibody [1D2-1C64].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200
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GTX31146 WB Image
WB analysis of 25 μg of various whole cell lysates per well using GTX31146 Rap1 antibody [1D2-1C64].
Dilution : 1:500
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GTX31146 IP Image
IP analysis of NIH3T3 cell lysates using GTX31146 Rap1 antibody [1D2-1C64].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:500
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GTX31146 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31146 Rap1 antibody [1D2-1C64].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature
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GTX31146 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX31146 Rap1 antibody [1D2-1C64]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
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REFERENCE
There are currently no references for Rap1 antibody [1D2-1C64] (GTX31146). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rap1 antibody [1D2-1C64] (GTX31146). Be the first to share your experience with this product.
![Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146](/upload/website/prouct_img/small/GTX31146/GTX31146_1345_IHC-P_w_23060722_488.webp "Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146](/upload/website/prouct_img/small/GTX31146/GTX31146_1854_WB_w_23060722_951.webp "Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146](/upload/website/prouct_img/small/GTX31146/GTX31146_1452_IP_w_23060722_403.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146](/upload/website/prouct_img/small/GTX31146/GTX31146_761_ICC-IF_w_23060722_502.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146](/upload/website/prouct_img/small/GTX31146/GTX31146_762_ICC-IF_w_23060722_853.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146](/upload/website/prouct_img/normal/GTX31146/GTX31146_1345_IHC-P_w_23060722_488.webp "Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146](/upload/website/prouct_img/normal/GTX31146/GTX31146_1854_WB_w_23060722_951.webp "Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146](/upload/website/prouct_img/normal/GTX31146/GTX31146_1452_IP_w_23060722_403.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146](/upload/website/prouct_img/normal/GTX31146/GTX31146_761_ICC-IF_w_23060722_502.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146")
![Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146](/upload/website/prouct_img/normal/GTX31146/GTX31146_762_ICC-IF_w_23060722_853.webp "Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146")