GeneTex
  • Country / Location Selection

United States (US)

Rap1 antibody [1D2-1C64]

Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146
Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146
Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146
Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146
Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146

Cat No. GTX31146

Host

Mouse

Clonality

Monoclonal

Clone Name

1D2-1C64

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:10-1:200
IHC-P 1:100-1:1000
IP 2 μg
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 30% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human Rap1

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RAP1A, member of RAS oncogene family , C21KG , G-22K , KREV-1 , KREV1 , RAP1 , SMGP21

Cellular Localization

Cell membrane,Cytoplasm,Cell junction

Background

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146

GTX31146 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31146 Rap1 antibody [1D2-1C64].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-Rap1 antibody [1D2-1C64] used in Immunoprecipitation (IP). GTX31146

GTX31146 IP Image

IP analysis of NIH3T3 cell lysates using GTX31146 Rap1 antibody [1D2-1C64].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:500

Anti-Rap1 antibody [1D2-1C64] used in IHC (Paraffin sections) (IHC-P). GTX31146

GTX31146 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31146 Rap1 antibody [1D2-1C64].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-Rap1 antibody [1D2-1C64] used in Western Blot (WB). GTX31146

GTX31146 WB Image

WB analysis of 25 μg of various whole cell lysates per well using GTX31146 Rap1 antibody [1D2-1C64].
Dilution : 1:500

Anti-Rap1 antibody [1D2-1C64] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31146

GTX31146 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX31146 Rap1 antibody [1D2-1C64]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

REFERENCE

There are currently no references for Rap1 antibody [1D2-1C64] (GTX31146). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Rap1 antibody [1D2-1C64] (GTX31146). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329