ICC/IF analysis of C2C12 cells using GTX31146 Rap1 antibody [1D2-1C64]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX31146 Rap1 antibody [1D2-1C64].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IP analysis of NIH3T3 cell lysates using GTX31146 Rap1 antibody [1D2-1C64].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:500

WB analysis of 25 μg of various whole cell lysates per well using GTX31146 Rap1 antibody [1D2-1C64].
Dilution : 1:500

IHC-P analysis of human kidney tissue using GTX31146 Rap1 antibody [1D2-1C64].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200