Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rap1GAP antibody [N2C2], Internal detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min