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Rap1GAP antibody

Anti-Rap1GAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX133357
Anti-Rap1GAP antibody used in IHC (Paraffin sections) (IHC-P). GTX133357
Anti-Rap1GAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133357
Anti-Rap1GAP antibody used in IHC (Paraffin sections) (IHC-P). GTX133357
Anti-Rap1GAP antibody used in Western Blot (WB). GTX133357

Cat. No. GTX133357

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rap1GAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Rap1GAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886953

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rap1 GTPase-activating protein , 1300019I11Rik , 2310004O14Rik , AI427470 , ARPP-90 , Gap , Rap1GAP1 , Rap1ga1

Cellular Localization

Golgi apparatus membrane

Database

Research Area

DATA IMAGES

Anti-Rap1GAP antibody used in IHC (Frozen sections) (IHC-Fr). GTX133357

GTX133357 IHC-Fr Image

Rap1GAP antibody detects Rap1GAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Rap1GAP protein stained by Rap1GAP antibody (GTX133357) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-Rap1GAP antibody used in IHC (Paraffin sections) (IHC-P). GTX133357

GTX133357 IHC-P Image

Rap1GAP antibody detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Rap1GAP antibody (GTX133357) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rap1GAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133357

GTX133357 ICC/IF Image

Rap1GAP antibody detects Rap1GAP protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rap1GAP stained by Rap1GAP antibody (GTX133357) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rap1GAP antibody used in IHC (Paraffin sections) (IHC-P). GTX133357

GTX133357 IHC-P Image

Rap1GAP antibody detects Rap1GAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Rap1GAP antibody (GTX133357) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rap1GAP antibody used in Western Blot (WB). GTX133357

GTX133357 WB Image

Various tissue extracts (10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody (GTX133357) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169