Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Raptor antibody (GTX132303) diluted at 1:1000.

Immunoprecipitation of Raptor protein from 293T whole cell extracts using 5 μg of Raptor antibody (GTX132303).
Western blot analysis was performed using Raptor antibody (GTX132303) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Raptor antibody detects Raptor protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Raptor antibody (GTX132303) diluted at 1:1500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min