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Raptor antibody

Anti-Raptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132303
Anti-Raptor antibody used in Immunoprecipitation (IP). GTX132303
Anti-Raptor antibody used in Western Blot (WB). GTX132303

Cat. No. GTX132303

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Raptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1500
IP 1:100-1:500
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Raptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulatory associated protein of MTOR complex 1 , KOG1 , Mip1

Background

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Raptor antibody used in IHC (Paraffin sections) (IHC-P). GTX132303

GTX132303 IHC-P Image

Raptor antibody detects Raptor protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Raptor antibody (GTX132303) diluted at 1:1500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Raptor antibody used in Immunoprecipitation (IP). GTX132303

GTX132303 IP Image

Immunoprecipitation of Raptor protein from 293T whole cell extracts using 5 μg of Raptor antibody (GTX132303).
Western blot analysis was performed using Raptor antibody (GTX132303) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Raptor antibody used in Western Blot (WB). GTX132303

GTX132303 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Raptor antibody (GTX132303) diluted at 1:1000.

REFERENCE

Application Reference

Chang CH et al. Oncotarget 2015; 6 (3) : 1478-89 Gab1 is essential for membrane translocation, activity and integrity of mTORCs after EGF stimulation in urothelial cell carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169