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Raptor antibody

KO/KD
Anti-Raptor antibody used in Western Blot (WB). GTX132989
Anti-Raptor antibody used in Western Blot (WB). GTX132989

Cat. No. GTX132989

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Raptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

Positive Control

293T , HepG2 , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886800

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulatory associated protein of MTOR complex 1 , KOG1 , Mip1

Background

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Raptor antibody used in Western Blot (WB). GTX132989

GTX132989 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Raptor antibody (GTX132989) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Raptor antibody used in Western Blot (WB). GTX132989

GTX132989 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raptor antibody (GTX132989) diluted at 1:1000.

REFERENCE

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REVIEW

Raptor antibody Cat. No. GTX132989

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-Raptor antibody used in Western Blot (WB). GTX132989
Date : Anonymous submitted on 13-Mar-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 15 μg
Blocking : 3% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169