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Raptor (phospho Ser792) antibody

Orthogonal
Anti-Raptor (phospho Ser792) antibody used in Western Blot (WB). GTX66614

Cat. No. GTX66614

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($429)
See all Raptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from human Raptor around the phosphorylation site of Ser792.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

regulatory associated protein of MTOR complex 1 , KOG1 , Mip1

Cellular Localization

Cytoplasm,Lysosome

Background

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Raptor (phospho Ser792) antibody used in Western Blot (WB). GTX66614

GTX66614 WB Image

Orthogonal

WB analysis of various samples using GTX66614 Raptor (phospho Ser792) antibody.
Lane1 : HEK293T whole cell lysate
Lane2 : HEK293T treated with Etoposide(20ng/ml) for 15 minutes whole cell lysate
Lane3 : HEK293T treated with Etoposide(20ng/ml) for 6 hours whole cell lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429