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Rat Arginase 1 protein (active)

Rat Arginase 1 protein (active). GTX00359-pro
Rat Arginase 1 protein (active). GTX00359-pro
Rat Arginase 1 protein (active). GTX00359-pro

Cat No. GTX00359-pro

Application Functional Assay
Species Rat
Package
10 μg ($279)
APPLICATION

Application Note

Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ERa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was in a dose dependent manner.

Observed MW

37 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Lys323 (NP_058830.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

arginase 1

Background

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Arginase 1 protein (active). GTX00359-pro

GTX00359-pro Functional Assay Image

Functional ELISA analysis of GTX00359-pro Rat Arginase 1 protein (active) which can bind immobilized ER alpha protein.

Rat Arginase 1 protein (active). GTX00359-pro

GTX00359-pro Image

WB analysis of GTX00359-pro Rat Arginase 1 protein (active).

Rat Arginase 1 protein (active). GTX00359-pro

GTX00359-pro Image

SDS-PAGE analysis of GTX00359-pro Rat Arginase 1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279