GeneTex
  • Country / Location Selection

United States (US)

Rat BDNF protein (active)

Rat BDNF protein (active). GTX00367-pro
Rat BDNF protein (active). GTX00367-pro
Rat BDNF protein (active). GTX00367-pro

Cat No. GTX00367-pro

Application Functional Assay
Species Rat
Package
10 μg ($319)
APPLICATION

Application Note

Brain-derived neurotrophic factor, also known as BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Amyloid Precursor Protein (APP) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat BDNF and recombinant rat APP. Briefly, BDNF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APP-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-BDNF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of BDNF and APP was in a dose dependent manner.

Observed MW

17 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn117~Gly248 (NP_001257559.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

brain-derived neurotrophic factor

Background

plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat BDNF protein (active). GTX00367-pro

GTX00367-pro Functional Assay Image

Functional ELISA analysis of GTX00367-pro Rat BDNF protein (active) which can bind immobilized APP protein.

Rat BDNF protein (active). GTX00367-pro

GTX00367-pro Image

WB analysis of GTX00367-pro Rat BDNF protein (active).

Rat BDNF protein (active). GTX00367-pro

GTX00367-pro Image

SDS-PAGE analysis of GTX00367-pro Rat BDNF protein (active).

REFERENCE
There are currently no references for Rat BDNF protein (active) (GTX00367-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rat BDNF protein (active) (GTX00367-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 319