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Rat CHI3L1 protein (active)

Rat CHI3L1 protein (active). GTX00364-pro
Rat CHI3L1 protein (active). GTX00364-pro
Rat CHI3L1 protein (active). GTX00364-pro

Cat No. GTX00364-pro

Application Functional Assay
Species Rat
Package
10 μg ($309)
APPLICATION

Application Note

Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1), YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 may play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. To test the effect of GP39 on cell proliferation, rat spleen cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 2% serum standard RPMI-1640 including various concentrations of recombinant rat GP39. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of spleen cells after incubation with GP39 for 96h observed by inverted microscope.

Observed MW

29 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr117~Lys364 (NP_001296749.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chitinase 3 like 1,CGP-39,Chil1,GP-39

Background

human homolog plays a role in vascular smooth muscle cell migration and adhesion [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat CHI3L1 protein (active). GTX00364-pro

GTX00364-pro Functional Assay Image

Cell proliferation effect of GTX00364-pro Rat CHI3L1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated rat spleen cells cultured in RPMI-1640 for 96hrs.
(B) Rat spleen cells cultured in RPMI-1640 stimulated with 10 ng/ml GP39 for 96hrs.

Rat CHI3L1 protein (active). GTX00364-pro

GTX00364-pro Image

SDS-PAGE analysis of GTX00364-pro Rat CHI3L1 protein (active).

Rat CHI3L1 protein (active). GTX00364-pro

GTX00364-pro Image

WB analysis of GTX00364-pro Rat CHI3L1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309