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Rat CNTF protein, His tag (active)

Rat CNTF protein, His tag (active). GTX66949-pro

Cat No. GTX66949-pro

Application

Functional Assay

Species

Rat
Package
50 μg ($359)

APPLICATION

Application Note

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is less or equal to 40 ng/ml.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 223 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ciliary neurotrophic factor

Background

a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat CNTF protein, His tag (active). GTX66949-pro

GTX66949-pro Image

3 μg of GTX66949-pro Rat CNTF protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf