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Rat CTGF protein (active)

Rat CTGF protein (active). GTX00375-pro
Rat CTGF protein (active). GTX00375-pro
Rat CTGF protein (active). GTX00375-pro
Rat CTGF protein (active). GTX00375-pro

Cat No. GTX00375-pro

Application Functional Assay
Species Rat
Package
10 μg ($239)
APPLICATION

Application Note

Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. Besides, Actin Beta (ACTb) has been identified as an interactor of CTGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CTGF and recombinant rat ACTb. Briefly, CTGF were diluted serially in PBS, with 0. 01% BSA (pH 7. 4). Duplicate samples of 100 μl were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CTGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of CTGF and ACTb was in a dose dependent manner.
To measure the effect of CTGF on cell adhesion, a general procedure performance as follows: 100 μl PBS containing recombinant CTGF were incubated overnight at 4ºC in 96-well ELISA plates. Wells were blocked with 200μl PBS containing 3% BSA and then incubated for 1h at 37 ºCwith 100 μl PBS containing approximately 5x10⁴ 3T3 cells. Adherent cells were then fixed for 15 min with 5% formaldehyde and non-adherent cells were removed by washing each well three times with PBS. The remaining cells were incubated with 0. 5% cristal violet for 10mins then counted at high magnification (×400) randomly.

Observed MW

35 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gln25~Ala347 (NP_071602.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cellular communication network factor 2 , CTGRP , Ctgf

Background

growth factor that may be a mediator of TGFbeta1-driven matrix production and normal skeletal remodeling [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat CTGF protein (active). GTX00375-pro

GTX00375-pro Functional Assay Image

Cell adhesion effect of GTX00375-pro Rat CTGF protein (active).
Upper: (A) 3T3 cells were seeded into the well without CTGF and incubated for 1hr at 37 ℃.
(B) 3T3 cells were seeded into the well containting CTGF 1 μg/ml and incubated for 1hr at 37 ℃.
Lower: The number of adhered 3T3 cells were incubated with 0.5% Crystal violet for 10mins then counted at high magnification (×400) randomly (five fields for each well).

Rat CTGF protein (active). GTX00375-pro

GTX00375-pro Functional Assay Image

Functional ELISA analysis of GTX00375-pro Rat CTGF protein (active) which can bind immobilized ACTb protein.

Rat CTGF protein (active). GTX00375-pro

GTX00375-pro Image

WB analysis of GTX00375-pro Rat CTGF protein (active).

Rat CTGF protein (active). GTX00375-pro

GTX00375-pro Image

SDS-PAGE analysis of GTX00375-pro Rat CTGF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239