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Rat CTRC protein, His tag

Rat CTRC protein, His tag. GTX00353-pro
Rat CTRC protein, His tag. GTX00353-pro
Rat CTRC protein, His tag. GTX00353-pro

Cat. No. GTX00353-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($299)

APPLICATION

Application Note

Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was in a dose dependent manner.

Observed MW

30 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val30~leu268 (NP_001071117.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chymotrypsin C

Database

Research Area

DATA IMAGES

Rat CTRC protein, His tag. GTX00353-pro

GTX00353-pro Functional Assay Image

Functional ELISA analysis of GTX00353-pro Rat CTRC protein which can bind immobilized uPAR protein.

Rat CTRC protein, His tag. GTX00353-pro

GTX00353-pro Image

WB analysis of GTX00353-pro Rat CTRC protein.

Rat CTRC protein, His tag. GTX00353-pro

GTX00353-pro Image

SDS-PAGE analysis of GTX00353-pro Rat CTRC protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299