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Rat CXCL2 / GRO beta protein (active)

Rat CXCL2 / GRO beta protein (active). GTX00351-pro
Rat CXCL2 / GRO beta protein (active). GTX00351-pro
Rat CXCL2 / GRO beta protein (active). GTX00351-pro

Cat No. GTX00351-pro

Application Functional Assay
Species Rat
Package
10 μg ($279)
APPLICATION

Application Note

Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was in a dose dependent manner.

Observed MW

41 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser32~Asn100 (NP_446099.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C motif chemokine ligand 2,Mip-2,Scyb2

Background

chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]

Database

DATA IMAGES
Rat CXCL2 / GRO beta protein (active). GTX00351-pro

GTX00351-pro Functional Assay Image

Functional ELISA analysis of GTX00351-pro Rat CXCL2 / GRO beta protein (active) which can bind immobilized DPP4 protein.

Rat CXCL2 / GRO beta protein (active). GTX00351-pro

GTX00351-pro Image

WB analysis of GTX00351-pro Rat CXCL2 / GRO beta protein (active).

Rat CXCL2 / GRO beta protein (active). GTX00351-pro

GTX00351-pro Image

SDS-PAGE analysis of GTX00351-pro Rat CXCL2 / GRO beta protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 279