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Rat CXCL2 / GRO beta protein, His and GST tag

Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro
Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro
Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro

Cat. No. GTX00351-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($299)
See all CXCL2 / GRO beta protein products

APPLICATION

Application Note

Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was in a dose dependent manner.

Observed MW

41 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser32~Asn100 (NP_446099.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine ligand 2 , Mip-2 , Scyb2

Background

chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro

GTX00351-pro Functional Assay Image

Functional ELISA analysis of GTX00351-pro Rat CXCL2 / GRO beta protein which can bind immobilized DPP4 protein.

Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro

GTX00351-pro Image

SDS-PAGE analysis of GTX00351-pro Rat CXCL2 / GRO beta protein.

Rat CXCL2 / GRO beta protein, His and GST tag. GTX00351-pro

GTX00351-pro Image

WB analysis of GTX00351-pro Rat CXCL2 / GRO beta protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299