GeneTex
  • Country / Location Selection

United States (US)

Rat CYP1A1 protein (active)

Rat CYP1A1 protein (active). GTX00355-pro
Rat CYP1A1 protein (active). GTX00355-pro
Rat CYP1A1 protein (active). GTX00355-pro

Cat No. GTX00355-pro

Application Functional Assay
Species Rat
Package
10 μg ($329)
APPLICATION

Application Note

Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was in a dose dependent manner.

Observed MW

62 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser251~His521 (NP_036672.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cytochrome P450, family 1, subfamily a, polypeptide 1,AHH,AHRR,CP11,CYP1,Cyp45c,Cypc45c,P-450MC,P1-450,P450-C,P450DX

Background

monooxygenase that plays a role in dioxin metabolism and detoxification which is 3-methylcholanthrene-inducible [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat CYP1A1 protein (active). GTX00355-pro

GTX00355-pro Functional Assay Image

Functional ELISA analysis of GTX00355-pro Rat CYP1A1 protein (active) which can bind immobilized HSPA4 protein.

Rat CYP1A1 protein (active). GTX00355-pro

GTX00355-pro Image

WB analysis of GTX00355-pro Rat CYP1A1 protein (active).

Rat CYP1A1 protein (active). GTX00355-pro

GTX00355-pro Image

SDS-PAGE analysis of GTX00355-pro Rat CYP1A1 protein (active).

REFERENCE
There are currently no references for Rat CYP1A1 protein (active) (GTX00355-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rat CYP1A1 protein (active) (GTX00355-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 329