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Rat Calpain 1 protein, His tag

Rat Calpain 1 protein, His tag. GTX00358-pro
Rat Calpain 1 protein, His tag. GTX00358-pro
Rat Calpain 1 protein, His tag. GTX00358-pro

Cat. No. GTX00358-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($319)
See all Calpain 1 protein products

APPLICATION

Application Note

Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was in a dose dependent manner.

Observed MW

35 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Tyr387~Asp694 (NP_062025.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calpain 1

Background

calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Calpain 1 protein, His tag. GTX00358-pro

GTX00358-pro Functional Assay Image

Functional ELISA analysis of GTX00358-pro Rat Calpain 1 protein which can bind immobilized STAT3 protein.

Rat Calpain 1 protein, His tag. GTX00358-pro

GTX00358-pro Image

WB analysis of GTX00358-pro Rat Calpain 1 protein.

Rat Calpain 1 protein, His tag. GTX00358-pro

GTX00358-pro Image

SDS-PAGE analysis of GTX00358-pro Rat Calpain 1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 319