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Rat Caspase 1 protein, His tag

Rat Caspase 1 protein, His tag. GTX00060-pro
Rat Caspase 1 protein, His tag. GTX00060-pro
Rat Caspase 1 protein, His tag. GTX00060-pro

Cat. No. GTX00060-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($349)
See all Caspase 1 protein products

APPLICATION

Application Note

Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was in a dose dependent manner.

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn119~Asp296

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caspase 1 , Ice , Il1bc

Background

apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Caspase 1 protein, His tag. GTX00060-pro

GTX00060-pro Functional Assay Image

Functional ELISA analysis of GTX00060-pro Rat Caspase 1 protein which can bind immobilized IL1A protein.

Rat Caspase 1 protein, His tag. GTX00060-pro

GTX00060-pro Image

WB analysis of GTX00060-pro Rat Caspase 1 protein.

Rat Caspase 1 protein, His tag. GTX00060-pro

GTX00060-pro Image

SDS-PAGE analysis of GTX00060-pro Rat Caspase 1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 349