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Rat Caspase 1 protein (active)

Rat Caspase 1 protein (active). GTX00060-pro
Rat Caspase 1 protein (active). GTX00060-pro
Rat Caspase 1 protein (active). GTX00060-pro

Cat No. GTX00060-pro

Application Functional Assay
Species Rat
Package
10 μg ($329)
APPLICATION

Application Note

Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was in a dose dependent manner.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn119~Asp296

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

caspase 1,Ice,Il1bc

Background

apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Caspase 1 protein (active). GTX00060-pro

GTX00060-pro Functional Assay Image

Functional ELISA analysis of GTX00060-pro Rat Caspase 1 protein (active) which can bind immobilized IL1A protein.

Rat Caspase 1 protein (active). GTX00060-pro

GTX00060-pro Image

WB analysis of GTX00060-pro Rat Caspase 1 protein (active).

Rat Caspase 1 protein (active). GTX00060-pro

GTX00060-pro Image

SDS-PAGE analysis of GTX00060-pro Rat Caspase 1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329