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Rat Cathepsin A protein (active)

Rat Cathepsin A protein (active). GTX00059-pro
Rat Cathepsin A protein (active). GTX00059-pro
Rat Cathepsin A protein (active). GTX00059-pro

Cat No. GTX00059-pro

Application Functional Assay
Species Rat
Package
10 μg ($319)
APPLICATION

Application Note

Cathepsin A (CTSA) is an enzyme that is classified both as a cathepsin and a carboxypeptidase. This gene encodes a glycoprotein that associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high-molecular-weight multimers. The formation of this complex provides a protective role for stability and activity. It is protective for β-galactosidase and neuraminidase. Besides, Neuraminidase (NEU) has been identified as an interactor of CTSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CTSA and recombinant rat NEU. Briefly, CTSA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NEU-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CTSA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CTSA and NEU was in a dose dependent manner.

Observed MW

57 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ala151~Val394

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cathepsin A,Ppgb

Database

DATA IMAGES
Rat Cathepsin A protein (active). GTX00059-pro

GTX00059-pro Functional Assay Image

Functional ELISA analysis of GTX00059-pro Rat Cathepsin A protein (active) which can bind immobilized NEU protein.

Rat Cathepsin A protein (active). GTX00059-pro

GTX00059-pro Image

SDS-PAGE analysis of GTX00059-pro Rat Cathepsin A protein (active).

Rat Cathepsin A protein (active). GTX00059-pro

GTX00059-pro Image

WB analysis of GTX00059-pro Rat Cathepsin A protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 319