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Rat Coagulation factor III/Tissue Factor protein (active)

Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro
Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro
Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro

Cat No. GTX00054-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($329)

APPLICATION

Application Note

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was in a dose dependent manner.

Observed MW

29 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly30~Glu252

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coagulation factor III, tissue factor

Background

an initiation factor for blood coagulation; plays a critical role in hepatic ischemic reperfusion injury [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro

GTX00054-pro Functional Assay Image

Functional ELISA analysis of GTX00054-pro Rat Coagulation factor III/Tissue Factor protein (active) which can bind immobilized EGF protein.

Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro

GTX00054-pro Image

WB analysis of GTX00054-pro Rat Coagulation factor III/Tissue Factor protein (active).

Rat Coagulation factor III/Tissue Factor protein (active). GTX00054-pro

GTX00054-pro Image

SDS-PAGE analysis of GTX00054-pro Rat Coagulation factor III/Tissue Factor protein (active).

REFERENCE

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REVIEW

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