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Rat Cystatin C protein, His tag (active)

Rat Cystatin C protein, His tag (active). GTX66964-pro

Cat No. GTX66964-pro

Application

Functional Assay

Species

Rat
Package
50 μg ($289)

APPLICATION

Application Note

The IC₅₀ value is < 1.0 nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25ºC.

Observed MW

13.5-18 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

C-terminal His-Tag; Length: 128 a.a. Sequence: GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystatin C , CYSC

Background

extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Cystatin C protein, His tag (active). GTX66964-pro

GTX66964-pro Image

3 μg of GTX66964-pro Rat Cystatin C protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf