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Rat EndoG protein (active)

Rat EndoG protein (active). GTX00057-pro
Rat EndoG protein (active). GTX00057-pro
Rat EndoG protein (active). GTX00057-pro

Cat No. GTX00057-pro

Application Functional Assay
Species Rat
Package
10 μg ($309)
APPLICATION

Application Note

ENDOG (Endonuclease G) is a mitochondrial enzyme that cleaves DNA at double-stranded (DG)n. (DC)n and at single-stranded (DC)n tracts. This protein also has ribonuclease (RNase) and RNase H activities. It has been reported that EndoG forms complexes with some other proteins, including HSP70 (Heat shock 70 kDa protein). Besides, mouse HSPA1A (Heat shock 70 kDa protein 1A) shares similarities with rat HSP1A1 in amino acid sequence with the identity of 98.44% . Thus, a binding ELISA assay was conducted to detect the association of recombinant rat ENDOG with recombinant mouse HSPA1A. Briefly, ENDOG were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSP1A1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ENDOG with HSP1A1 was in a dose dependent manner.

Observed MW

62 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His and GST-Tag; Met1~Lys294 (NP_001030110.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

endonuclease G

Database

DATA IMAGES
Rat EndoG protein (active). GTX00057-pro

GTX00057-pro Functional Assay Image

Functional ELISA analysis of GTX00057-pro Rat EndoG protein (active) which can bind immobilized HSP1A1 protein.

Rat EndoG protein (active). GTX00057-pro

GTX00057-pro Image

WB analysis of GTX00057-pro Rat EndoG protein (active).

Rat EndoG protein (active). GTX00057-pro

GTX00057-pro Image

SDS-PAGE analysis of GTX00057-pro Rat EndoG protein (active).

REFERENCE
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Package List Price ($)
$ 309