The chemotactic effect of GTX00345-pro Rat Endothelin 1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 80 ng/ml EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

WB analysis of GTX00345-pro Rat Endothelin 1 protein (active).

SDS-PAGE analysis of GTX00345-pro Rat Endothelin 1 protein (active).