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Rat Endothelin 1 protein (active)

Rat Endothelin 1 protein (active). GTX00345-pro
Rat Endothelin 1 protein (active). GTX00345-pro
Rat Endothelin 1 protein (active). GTX00345-pro

Cat No. GTX00345-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($319)

APPLICATION

Application Note

Endothelins (EDN) are small (21 amino acids) vasoactive peptides produced by many cell types including endothelial and epithelial cells, macrophages and fibroblasts. By binding to G-protein-linked transmembrane receptors, EDNs participate in vasoconstriction modulation and cell growth regulation. It has been proven that EDN1 has chemotaxis active on monocytes, thus chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of EDN1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶cells/ml in RPMI-1640 with 0. 5% FBS) and EDN1 (20 ng/ml, 40 ng/ml and 80 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly. EDN1 is able to induce migration of THP-1 cells, and the optimum chemotaxis of EDN1 occurs at 80 ng/ml.

Observed MW

19 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser54~His202 (NP_036680.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

endothelin 1 , Et1

Background

activates ERK1/2 mediated signaling; may play a role in regulation of cardiac contractile force [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Functional Assay Image

The chemotactic effect of GTX00345-pro Rat Endothelin 1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 80 ng/ml EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Image

SDS-PAGE analysis of GTX00345-pro Rat Endothelin 1 protein (active).

Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Image

WB analysis of GTX00345-pro Rat Endothelin 1 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 319