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Rat Endothelin 1 protein (active)

Rat Endothelin 1 protein (active). GTX00345-pro
Rat Endothelin 1 protein (active). GTX00345-pro
Rat Endothelin 1 protein (active). GTX00345-pro

Cat No. GTX00345-pro

Application Functional Assay
Species Rat
Package
10 μg ($319)
APPLICATION

Application Note

Endothelins (EDN) are small (21 amino acids) vasoactive peptides produced by many cell types including endothelial and epithelial cells, macrophages and fibroblasts. By binding to G-protein-linked transmembrane receptors, EDNs participate in vasoconstriction modulation and cell growth regulation. It has been proven that EDN1 has chemotaxis active on monocytes, thus chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of EDN1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶cells/ml in RPMI-1640 with 0. 5% FBS) and EDN1 (20 ng/ml, 40 ng/ml and 80 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly. EDN1 is able to induce migration of THP-1 cells, and the optimum chemotaxis of EDN1 occurs at 80 ng/ml.

Observed MW

19 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser54~His202 (NP_036680.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

endothelin 1,Et1

Background

activates ERK1/2 mediated signaling; may play a role in regulation of cardiac contractile force [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Functional Assay Image

The chemotactic effect of GTX00345-pro Rat Endothelin 1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 80 ng/ml EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Image

SDS-PAGE analysis of GTX00345-pro Rat Endothelin 1 protein (active).

Rat Endothelin 1 protein (active). GTX00345-pro

GTX00345-pro Image

WB analysis of GTX00345-pro Rat Endothelin 1 protein (active).

REFERENCE
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Package List Price ($)
$ 319