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Rat Factor VII protein (active)

Rat Factor VII protein (active). GTX00352-pro
Rat Factor VII protein (active). GTX00352-pro
Rat Factor VII protein (active). GTX00352-pro

Cat No. GTX00352-pro

Application Functional Assay
Species Rat
Package
10 μg ($339)
APPLICATION

Application Note

The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels-normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating FVII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Tissue Factor (TF) has been identified as an interactor of FVII, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FVII and recombinant rat TF. Briefly, FVII were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TF-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-FVII pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of FVII and TF was in a dose dependent manner.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala315~Asp433 (NP_690059.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Rat Factor VII protein (active). GTX00352-pro

GTX00352-pro Functional Assay Image

Functional ELISA analysis of GTX00352-pro Rat Factor VII protein (active) which can bind immobilized TF protein.

Rat Factor VII protein (active). GTX00352-pro

GTX00352-pro Image

SDS-PAGE analysis of GTX00352-pro Rat Factor VII protein (active).

Rat Factor VII protein (active). GTX00352-pro

GTX00352-pro Image

WB analysis of GTX00352-pro Rat Factor VII protein (active).

REFERENCE
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