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Rat GADD45A protein (active)

Rat GADD45A protein (active). GTX00066-pro
Rat GADD45A protein (active). GTX00066-pro
Rat GADD45A protein (active). GTX00066-pro

Cat No. GTX00066-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($289)

APPLICATION

Application Note

Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45a) is a member of the GADD45 family. It may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Besides, Proliferating Cell Nuclear Antigen (PCNA) has been identified as an interactor of GADD45a, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GADD45a and recombinant rat PCNA. Briefly, GADD45a were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PCNA-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GADD45a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of GADD45a and PCNA was in a dose dependent manner.

Observed MW

22 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Met1~Arg165

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

growth arrest and DNA-damage-inducible, alpha , Ddit1 , Gadd45

Background

may mediate a delay in G2 to M cell cycle progression; may induce DNA repair [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat GADD45A protein (active). GTX00066-pro

GTX00066-pro Functional Assay Image

Functional ELISA analysis of GTX00066-pro Rat GADD45A protein (active) which can bind immobilized PCNA protein.

Rat GADD45A protein (active). GTX00066-pro

GTX00066-pro Image

SDS-PAGE analysis of GTX00066-pro Rat GADD45A protein (active).

Rat GADD45A protein (active). GTX00066-pro

GTX00066-pro Image

WB analysis of GTX00066-pro Rat GADD45A protein (active).

REFERENCE

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REVIEW

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