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Rat GDF8 / Myostatin protein, His tag

Rat GDF8 / Myostatin protein, His tag. GTX00360-pro
Rat GDF8 / Myostatin protein, His tag. GTX00360-pro
Rat GDF8 / Myostatin protein, His tag. GTX00360-pro

Cat. No. GTX00360-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($309)
See all GDF8 / Myostatin protein products

APPLICATION

Application Note

Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat MSTN and recombinant rat FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was in a dose dependent manner.

Observed MW

14 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg267~Ser376 (NP_062024.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myostatin , Gdf8

Background

This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Jun 2016]

Database

Research Area

DATA IMAGES

Rat GDF8 / Myostatin protein, His tag. GTX00360-pro

GTX00360-pro Functional Assay Image

Functional ELISA analysis of GTX00360-pro Rat GDF8 / Myostatin protein which can bind immobilized FSTL3 protein.

Rat GDF8 / Myostatin protein, His tag. GTX00360-pro

GTX00360-pro Image

SDS-PAGE analysis of GTX00360-pro Rat GDF8 / Myostatin protein.

Rat GDF8 / Myostatin protein, His tag. GTX00360-pro

GTX00360-pro Image

WB analysis of GTX00360-pro Rat GDF8 / Myostatin protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309