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Rat GM-CSF protein (active)

Rat GM-CSF protein (active). GTX00365-pro
Rat GM-CSF protein (active). GTX00365-pro
Rat GM-CSF protein (active). GTX00365-pro

Cat No. GTX00365-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. It also has some effects on mature cells of the immune system. These include, for example, inhibiting neutrophil migration and causing an alteration of the receptors expressed on the cells surface. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Besides, Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) has been identified as an interactor of GM-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GM-CSF and recombinant rat CSF2Ra. Briefly, GM-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CSF2Ra-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-GM-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of GM-CSF and CSF2Ra was in a dose dependent manner.

Observed MW

45 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala18~Lys144 (NP_446304.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

colony stimulating factor 2,Gm-csf,Gmcsf

Background

plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat GM-CSF protein (active). GTX00365-pro

GTX00365-pro Functional Assay Image

Functional ELISA analysis of GTX00365-pro Rat GM-CSF protein (active) which can bind immobilized CSF2Ra protein.

Rat GM-CSF protein (active). GTX00365-pro

GTX00365-pro Image

WB analysis of GTX00365-pro Rat GM-CSF protein (active).

Rat GM-CSF protein (active). GTX00365-pro

GTX00365-pro Image

SDS-PAGE analysis of GTX00365-pro Rat GM-CSF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289