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Rat Galectin 3 protein, His tag (active)

Rat Galectin 3 protein, His tag (active). GTX00361-pro
Rat Galectin 3 protein, His tag (active). GTX00361-pro
Rat Galectin 3 protein, His tag (active). GTX00361-pro

Cat. No. GTX00361-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($299)
See all Galectin 3 protein products

APPLICATION

Application Note

Galectin 3 (GAL3) is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. The protein also has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. GAL3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. It also can agglutinate red blood. In this case, we choose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (HA) is as follows, two-fold dilute the recombinant rat GAL3 with 0. 01M PBS (pH 7. 4), add 50 μl a serial dilution of GAL3 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL3, replace with 50 μl 0. 01M PBS. Then add 50 μl 1% RaE to each well and mixed gently. The plate is incubated for 1-2 hours at room temperature. The minimal effective concentration of GAL3 is 2. 5μg/ml.

Observed MW

28 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Ile262 (NP_114020.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , AGE-R3 , CBP30 , L-34 , gal-3

Background

lectin molecule that binds IgE; may have role in immune function [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Galectin 3 protein, His tag (active). GTX00361-pro

GTX00361-pro Functional Assay Image

The hemagglutination effect of GTX00361-pro Rat Galectin 3 protein (active).
(A) 1% rabbit erythrocyte treate without GAL3.
(B) 1% rabbit erythrocyte treated with 2.5 μg/ml GAL3 for 2hrs.

Rat Galectin 3 protein, His tag (active). GTX00361-pro

GTX00361-pro Image

SDS-PAGE analysis of GTX00361-pro Rat Galectin 3 protein (active).

Rat Galectin 3 protein, His tag (active). GTX00361-pro

GTX00361-pro Image

WB analysis of GTX00361-pro Rat Galectin 3 protein (active).

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SDS
Proclin.pdf
Package List Price ($)
$ 299