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Rat Gremlin 1 protein (active)

Rat Gremlin 1 protein (active). GTX00362-pro
Rat Gremlin 1 protein (active). GTX00362-pro
Rat Gremlin 1 protein (active). GTX00362-pro

Cat No. GTX00362-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant rat GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GREM1 for 96h. And GREM1 significantly increased cell viability of A549 cells.

Observed MW

24 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys25~Asp184 (NP_062155.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

gremlin 1, DAN family BMP antagonist,Cktsf1b1,drm

Background

protein may play a role in cellular growth control, viability and differentiation; high expression levels may induce apoptosis [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Gremlin 1 protein (active). GTX00362-pro

GTX00362-pro Functional Assay Image

Cell proliferation effect of GTX00362-pro Rat Gremlin 1 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated A549 cells cultured in DMEM for 96hrs.
(B) A549 cells cultured in DMEM, stimulated with 1 ng/ml GREM1 for 96hrs.

Rat Gremlin 1 protein (active). GTX00362-pro

GTX00362-pro Image

WB analysis of GTX00362-pro Rat Gremlin 1 protein (active).

Rat Gremlin 1 protein (active). GTX00362-pro

GTX00362-pro Image

SDS-PAGE analysis of GTX00362-pro Rat Gremlin 1 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289