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Rat IL1 Receptor 1 protein, His tag

Rat IL1 Receptor 1 protein, His tag. GTX00053-pro
Rat IL1 Receptor 1 protein, His tag. GTX00053-pro
Rat IL1 Receptor 1 protein, His tag. GTX00053-pro

Cat. No. GTX00053-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($239)
See all IL1 Receptor 1 protein products

APPLICATION

Application Note

IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was in a dose dependent manner.

Observed MW

21 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp386~Tyr553

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 receptor type 1

Background

receptor for interleukin-1; involved in inflammatory response signalling [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat IL1 Receptor 1 protein, His tag. GTX00053-pro

GTX00053-pro Functional Assay Image

Functional ELISA analysis of GTX00053-pro Rat IL1 Receptor 1 protein which can bind immobilized IL1B protein.

Rat IL1 Receptor 1 protein, His tag. GTX00053-pro

GTX00053-pro Image

SDS-PAGE analysis of GTX00053-pro Rat IL1 Receptor 1 protein.

Rat IL1 Receptor 1 protein, His tag. GTX00053-pro

GTX00053-pro Image

WB analysis of GTX00053-pro Rat IL1 Receptor 1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 239