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Rat IL13 protein (active)

Rat IL13 protein (active). GTX00368-pro
Rat IL13 protein (active). GTX00368-pro
Rat IL13 protein (active). GTX00368-pro

Cat No. GTX00368-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Interleukin 13 (IL13) is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells. IL13 has effects on immune cells that are similar to those of the closely related cytokine IL4. Although IL13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL13 induces a class of protein-degrading enzymes, known as matrix metalloproteinases (MMPs), in the airways. Furthermore, IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells. Besides, Interleukin 13 Receptor Alpha 1 (IL13Ra1) has been identified as an interactor of IL13, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL13 and recombinant rat IL13Ra1. Briefly, IL13 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13Ra1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL13 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL13 and IL13Ra1 was in a dose dependent manner.

Observed MW

16 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 100 mM NaHCO₃ (pH 8.3) with 500 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 100 mM NaHCO₃ and 500 mM NaCl (pH 8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro22~His131 (NP_446280.1)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 13

Background

cytokine; involved in inflammatory and immune responses [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat IL13 protein (active). GTX00368-pro

GTX00368-pro Functional Assay Image

Functional ELISA analysis of GTX00368-pro Rat IL13 protein (active) which can bind immobilized IL13Ra1 protein.

Rat IL13 protein (active). GTX00368-pro

GTX00368-pro Image

WB analysis of GTX00368-pro Rat IL13 protein (active).

Rat IL13 protein (active). GTX00368-pro

GTX00368-pro Image

SDS-PAGE analysis of GTX00368-pro Rat IL13 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289