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Rat IL4 protein, His and GST tag (active)

Rat IL4 protein, His and GST tag (active). GTX00366-pro
Rat IL4 protein, His and GST tag (active). GTX00366-pro
Rat IL4 protein, His and GST tag (active). GTX00366-pro

Cat No. GTX00366-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($279)

APPLICATION

Application Note

The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL4 and recombinant rat IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was in a dose dependent manner.

Observed MW

36 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Asn61~Leu111 (NP_958427.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 4 , Il4e12

Background

Th2-type cytokine; may be involved in inflammatory response in eosinophils [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat IL4 protein, His and GST tag (active). GTX00366-pro

GTX00366-pro Functional Assay Image

Functional ELISA analysis of GTX00366-pro Rat IL4 protein (active) which can bind immobilized IL2Rg protein.

Rat IL4 protein, His and GST tag (active). GTX00366-pro

GTX00366-pro Image

WB analysis of GTX00366-pro Rat IL4 protein (active).

Rat IL4 protein, His and GST tag (active). GTX00366-pro

GTX00366-pro Image

SDS-PAGE analysis of GTX00366-pro Rat IL4 protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 279