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Rat IL6 protein (active)

Rat IL6 protein (active). GTX00341-pro
Rat IL6 protein (active). GTX00341-pro
Rat IL6 protein (active). GTX00341-pro

Cat No. GTX00341-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($259)

APPLICATION

Application Note

Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot. MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6.

Observed MW

27 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.6) with 5% trehalose. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe25~Thr211 (NP_036721.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , ILg6 , Ifnb2

Background

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat IL6 protein (active). GTX00341-pro

GTX00341-pro Functional Assay Image

ELISA detection of secreted MMP10 protein activation from A549 cells induced by GTX00341-pro Rat IL6 protein (active) for 24hrs.
Recombinant protein concentrations: 25, and 50 ng/ml.

Rat IL6 protein (active). GTX00341-pro

GTX00341-pro Image

SDS-PAGE analysis of GTX00341-pro Rat IL6 protein (active).

Rat IL6 protein (active). GTX00341-pro

GTX00341-pro Image

WB analysis of GTX00341-pro Rat IL6 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 259