Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Keyhole limpet hemocyanin (KLH)
Conjugation
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
monoclonal Rat IgG1 isotype control , Rat IgG1 kappa isotype control , Rat isotype , Rat IgG1 kappa negative control , Isotype control to Rat IgG1 kappa , Isotype control for Rat IgG1 kappa , Rat IgG1 negative control , Rat negative control for IgG1 kappa
Background
Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).