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Rat IgG2a isotype control [KLH/G2a-1-1] (Biotin)

Cat No. GTX35057

Host Rat
Clonality Monoclonal
Isotype IgG2a
Application FACS, ELISA
Package
100 μg ($129)
APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Specificity/Sensitivity

Keyhole limpet hemocyanin (KLH)
PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% NaN3

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keyhole limpet hemocyanin (KLH)

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Isotype control for Rat IgG2a kappa, Rat IgG2a negative control, Rat negative control for IgG2a kappa, Rat IgG2a kappa isotype control, Rat isotype control for IgG2a kappa, Negative contr, Rat IgG2a isotype control, Rat IgG2a kappa negative control

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
REFERENCE
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REVIEW
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Package List Price ($)
$ 129