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Rat IgG2b isotype control [KLH/G2b-1-2] (FITC)

Cat No. GTX35060

Host Rat
Clonality Monoclonal
Clone Name KLH/G2b-1-2
Isotype IgG2b
Application FACS, ELISA
Package
50 μg ($99)
APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Specificity/Sensitivity

Keyhole limpet hemocyanin (KLH)
PROPERTIES

Form

Liquid

Buffer

PBS + 0.1% NaN3

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keyhole limpet hemocyanin (KLH)

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Negative control for Rat IgG2b kappa, monoclonal Rat IgG2b isotype control, Isotype control for Rat IgG2b kappa, Rat IgG2b kappa negative control, Rat IgG2b isotype control, Rat IgG2b negative control, Rat negative control for IgG2b kappa, Isotype

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
REFERENCE
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REVIEW
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Package List Price ($)
$ 99