APPLICATION
Application Note
Use at a concentration/dilution equivalent to that of your experimental primary antibody.
Specificity/Sensitivity
Keyhole limpet hemocyanin (KLH)
PROPERTIES
Form
Liquid
Buffer
PBS + 0.1% NaN3
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Keyhole limpet hemocyanin (KLH)
Conjugation
Biotin
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Rat IgG2c isotype control, Isotype control to Rat IgG2c kappa, Rat IgG2c kappa negative control, monoclonal Rat IgG2c isotype control, Isotype control for Rat IgG2c kappa, Rat negative control for IgG2c kappa, Negative control for Rat IgG2c kappa
Background
Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
REFERENCE
There are currently no references for Rat IgG2c isotype control [SB68b] (Biotin) (GTX35064). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rat IgG2c isotype control [SB68b] (Biotin) (GTX35064). Be the first to share your experience with this product.
