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Rat IgG2c isotype control [SB68b]

Cat No. GTX35063

Host

Rat

Clonality

Monoclonal

Clone Name

SB68b

Isotype

IgG2c

Application

WB, FACS, IP, ELISA
Package
100 μg ($79)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Specificity/Sensitivity

Keyhole limpet hemocyanin (KLH)

PROPERTIES

Form

Liquid

Buffer

100 mM borate buffered saline, pH 8.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Keyhole limpet hemocyanin (KLH)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Rat IgG2c isotype control, Isotype control to Rat IgG2c kappa, Rat IgG2c kappa negative control, monoclonal Rat IgG2c isotype control, Isotype control for Rat IgG2c kappa, Rat negative control for IgG2c kappa, Negative control for Rat IgG2c kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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Package List Price ($)
$ 79