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Rat IgM isotype control [NIP/M-2] (FITC)

Cat. No. GTX35075

Host

 Rat

Clonality

 Monoclonal

Clone Name

 NIP/M-2

Isotype

 IgM

Application

 FACS, ELISA
Package
50 μg ($99)
See all Rat IgM isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Product Note

NIP

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

NIP

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

monoclonal Rat IgM isotype control , Negative control for Rat IgM , Isotype control for Rat IgM , Rat IgM isotype control , Rat isotype control for IgM , Rat IgM negative control , Rat negative control for IgM , Isotype control to Rat IgM

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 99