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Rat Interferon gamma protein, His tag (active)

Rat Interferon gamma protein, His tag (active). GTX00340-pro
Rat Interferon gamma protein, His tag (active). GTX00340-pro
Rat Interferon gamma protein, His tag (active). GTX00340-pro
Rat Interferon gamma protein, His tag (active). GTX00340-pro

Cat. No. GTX00340-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($209)
See all Interferon gamma protein products

APPLICATION

Application Note

Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264. 7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264. 7 cells were incubated in DMEM with IFN-γ (10 ng/ml) for 24h, then cells were observed by inverted microscope.

Observed MW

16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gln23~Cys156 (NP_620235.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon gamma , IFNG2

Background

an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Interferon gamma protein, His tag (active). GTX00340-pro

GTX00340-pro Functional Assay Image

Activation effect of GTX00340-pro Rat Interferon gamma protein (active).
(A) Unstimulated Raw 264.7 cells cultured in DMEM.
(B) Raw 264.7 cells cultured in DMEM, stimulated with IFN gamma.

Rat Interferon gamma protein, His tag (active). GTX00340-pro

GTX00340-pro Functional Assay Image

ELISA detection of secreted iNOS expression from RAW 264.7 cells stimulated by GTX00340-pro Rat Interferon gamma protein (active) at 10 ng/ml for 24hrs.

Rat Interferon gamma protein, His tag (active). GTX00340-pro

GTX00340-pro Image

SDS-PAGE analysis of GTX00340-pro Rat Interferon gamma protein (active).

Rat Interferon gamma protein, His tag (active). GTX00340-pro

GTX00340-pro Image

WB analysis of GTX00340-pro Rat Interferon gamma protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 209