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Rat Klotho protein (active)

Rat Klotho protein (active). GTX00378-pro
Rat Klotho protein (active). GTX00378-pro
Rat Klotho protein (active). GTX00378-pro

Cat No. GTX00378-pro

Application Functional Assay
Species Rat
Package
10 μg ($279)
APPLICATION

Application Note

Klotho (KL) is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. The Klotho protein is a novel β-glucuronidase capable of hydrolyzing steroid β-glucuronides. Genetic variants in KLOTHO have been associated with human aging, and Klotho protein has been shown to be a circulating factor detectable in serum that declines with age. The binding of certain fibroblast growth factors (FGF's) viz., FGF19, FGF20, and FGF23, to their fibroblast growth factor receptors, is promoted via their interactions as co-receptors with β-Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) has been identified as an interactor of KL, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGF23-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-KL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of KL and FGF23 was in a dose dependent manner.

Observed MW

55 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu517~Leu956 (NP_112626.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Klotho

Background

may be involved in suprresion of aging phenotypes [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Klotho protein (active). GTX00378-pro

GTX00378-pro Functional Assay Image

Functional ELISA analysis of GTX00378-pro Rat Klotho protein (active) which can bind immobilized FGF23 protein.

Rat Klotho protein (active). GTX00378-pro

GTX00378-pro Image

WB analysis of GTX00378-pro Rat Klotho protein (active).

Rat Klotho protein (active). GTX00378-pro

GTX00378-pro Image

SDS-PAGE analysis of GTX00378-pro Rat Klotho protein (active).

REFERENCE
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REVIEW
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