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Rat LDHA protein, His tag (active)

Rat LDHA protein, His tag (active). GTX67083-pro

Cat No. GTX67083-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($219)

APPLICATION

Application Note

Specific activity is > 200 units/mg, in which one unit will convert 1.0 μmole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37ºC.

Observed MW

28-40 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4) with 1 mM DTT, 20% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml

Region/Sequence

Full length protein, C-terminal His-Tag; Length: 340 a.a. Sequence: MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lactate dehydrogenase A , CDK1 , Ldh1

Background

mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat LDHA protein, His tag (active). GTX67083-pro

GTX67083-pro Image

3 μg of GTX67083-pro Rat LDHA protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf