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Rat Noggin protein, His and GST tag

Rat Noggin protein, His and GST tag. GTX00056-pro
Rat Noggin protein, His and GST tag. GTX00056-pro
Rat Noggin protein, His and GST tag. GTX00056-pro

Cat. No. GTX00056-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($299)
See all Noggin protein products

APPLICATION

Application Note

NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was in a dose dependent manner.

Observed MW

44 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Met28~Cys144

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

noggin

Background

secreted protein; involved in dorsal fate specification [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Noggin protein, His and GST tag. GTX00056-pro

GTX00056-pro Functional Assay Image

Functional ELISA analysis of GTX00056-pro Rat Noggin protein which can bind immobilized BMP4 protein.

Rat Noggin protein, His and GST tag. GTX00056-pro

GTX00056-pro Image

WB analysis of GTX00056-pro Rat Noggin protein.

Rat Noggin protein, His and GST tag. GTX00056-pro

GTX00056-pro Image

SDS-PAGE analysis of GTX00056-pro Rat Noggin protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299