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Rat ODC protein (active)

Rat ODC protein (active). GTX00369-pro
Rat ODC protein (active). GTX00369-pro
Rat ODC protein (active). GTX00369-pro

Cat No. GTX00369-pro

Application

Functional Assay

Species

Rat
Package
10 μg ($319)

APPLICATION

Application Note

The enzyme ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine (a product of the urea cycle) to form putrescine. This reaction is the committed step in polyamine synthesis. Lysine 69 on ornithine decarboxylase (ODC) binds the cofactor pyridoxal phosphate to form a Schiff base. Ornithine displaces the lysine to form a Schiff base attached to ODC, which decarboxylates to form a quinoid intermediate. This intermediate rearranges to form a Schiff base attached to putrescine, which is attacked by lysine to release putrescine product and reform PLP-bound ODC. Besides, Thymidine Kinase 1, Soluble (TK1) has been identified as an interactor of ODC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ODC and recombinant rat TK1. Briefly, ODC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TK1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-ODC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of ODC and TK1 was in a dose dependent manner.

Observed MW

55 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

Full length protein, N-terminal His-Tag; Met1~Val461 (NP_001289012.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ornithine decarboxylase 1 , Odc

Background

catalyzes the conversion of L-ornithine to putrescine [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat ODC protein (active). GTX00369-pro

GTX00369-pro Functional Assay Image

Functional ELISA analysis of GTX00369-pro Rat ODC protein (active) which can bind immobilized TK1 protein.

Rat ODC protein (active). GTX00369-pro

GTX00369-pro Image

SDS-PAGE analysis of GTX00369-pro Rat ODC protein (active).

Rat ODC protein (active). GTX00369-pro

GTX00369-pro Image

WB analysis of GTX00369-pro Rat ODC protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 319