GeneTex

  • Country / Location Selection

United States (US)

Rat Osteopontin protein, His tag (active)

Rat Osteopontin protein, His tag (active). GTX00349-pro
Rat Osteopontin protein, His tag (active). GTX00349-pro
Rat Osteopontin protein, His tag (active). GTX00349-pro

Cat. No. GTX00349-pro

Application

 Functional Assay

Species

 Rat
Package
10 μg ($349)
See all Osteopontin protein products

APPLICATION

Application Note

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1×10⁵ cells/ml, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Observed MW

60 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu17~Asn317 (NP_037013.2)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secreted phosphoprotein 1 , OSP

Background

sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Rat Osteopontin protein, His tag (active). GTX00349-pro

GTX00349-pro Functional Assay Image

ELISA detection of secreted MCP-1 protein activation from MCF-7 cells induced by GTX00349-pro Rat Osteopontin protein (active) for 24hrs.
Recombinant protein concentrations: 100, 200, and 400 ng/ml.

Rat Osteopontin protein, His tag (active). GTX00349-pro

GTX00349-pro Image

WB analysis of GTX00349-pro Rat Osteopontin protein (active).

Rat Osteopontin protein, His tag (active). GTX00349-pro

GTX00349-pro Image

SDS-PAGE analysis of GTX00349-pro Rat Osteopontin protein (active).

REFERENCE

There are currently no references for Rat Osteopontin protein, His tag (active) (GTX00349-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Rat Osteopontin protein, His tag (active) (GTX00349-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 349