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Rat Osteopontin protein (active)

Rat Osteopontin protein (active). GTX00349-pro
Rat Osteopontin protein (active). GTX00349-pro
Rat Osteopontin protein (active). GTX00349-pro

Cat No. GTX00349-pro

Application Functional Assay
Species Rat
Package
10 μg ($329)
APPLICATION

Application Note

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1×10⁵ cells/ml, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Observed MW

60 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu17~Asn317 (NP_037013.2)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

secreted phosphoprotein 1,OSP

Background

sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Functional Assay Image

ELISA detection of secreted MCP-1 protein activation from MCF-7 cells induced by GTX00349-pro Rat Osteopontin protein (active) for 24hrs.
Recombinant protein concentrations: 100, 200, and 400 ng/ml.

Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Image

WB analysis of GTX00349-pro Rat Osteopontin protein (active).

Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Image

SDS-PAGE analysis of GTX00349-pro Rat Osteopontin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329