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Rat Osteopontin protein (active)

Rat Osteopontin protein (active). GTX00349-pro
Rat Osteopontin protein (active). GTX00349-pro
Rat Osteopontin protein (active). GTX00349-pro

Cat No. GTX00349-pro

Application Functional Assay
Species Rat
Package
10 μg ($329)
APPLICATION

Application Note

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1×10⁵ cells/ml, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Observed MW

60 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu17~Asn317 (NP_037013.2)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

secreted phosphoprotein 1 , OSP

Background

sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Functional Assay Image

ELISA detection of secreted MCP-1 protein activation from MCF-7 cells induced by GTX00349-pro Rat Osteopontin protein (active) for 24hrs.
Recombinant protein concentrations: 100, 200, and 400 ng/ml.

Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Image

WB analysis of GTX00349-pro Rat Osteopontin protein (active).

Rat Osteopontin protein (active). GTX00349-pro

GTX00349-pro Image

SDS-PAGE analysis of GTX00349-pro Rat Osteopontin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 329