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Rat Parathyroid Hormone protein (active)

Rat Parathyroid Hormone protein (active). GTX00348-pro
Rat Parathyroid Hormone protein (active). GTX00348-pro
Rat Parathyroid Hormone protein (active). GTX00348-pro

Cat No. GTX00348-pro

Application Functional Assay
Species Rat
Package
10 μg ($249)
APPLICATION

Application Note

PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2. 8, UMR1x10⁶, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71. 3% . Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly,  U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of PTH. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. And PTH increased cell viability of U2OS cells.

Observed MW

59 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and MBP-Tag; Ala32~Gln115 (NP_058740.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

parathyroid hormone,PTH-(1-84),Pth1,Pthr1

Background

regulates osteoblast proliferation and differentiation; involved in G-protein coupled receptor mediated adenylate cyclase activation [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat Parathyroid Hormone protein (active). GTX00348-pro

GTX00348-pro Functional Assay Image

Cell proliferation effect of GTX00348-pro Rat Parathyroid Hormone protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated U2OS cells cultured in DMEM for 48hrs.
(B) U2OS cells cultured in DMEM, stimulated with 1 ng/ml PTH for 48hrs.

Rat Parathyroid Hormone protein (active). GTX00348-pro

GTX00348-pro Image

SDS-PAGE analysis of GTX00348-pro Rat Parathyroid Hormone protein (active).

Rat Parathyroid Hormone protein (active). GTX00348-pro

GTX00348-pro Image

WB analysis of GTX00348-pro Rat Parathyroid Hormone protein (active).

REFERENCE
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Package List Price ($)
$ 249