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Rat Plasminogen protein (active)

Rat Plasminogen protein (active). GTX00354-pro
Rat Plasminogen protein (active). GTX00354-pro
Rat Plasminogen protein (active). GTX00354-pro

Cat No. GTX00354-pro

Application Functional Assay
Species Rat
Package
10 μg ($289)
APPLICATION

Application Note

Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was in a dose dependent manner.

Observed MW

38 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Glu191~Arg433 (NP_445943.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

plasminogen,Ab1-346

Database

Research Area

DATA IMAGES
Rat Plasminogen protein (active). GTX00354-pro

GTX00354-pro Functional Assay Image

Functional ELISA analysis of GTX00354-pro Rat Plasminogen protein (active) which can bind immobilized ACT beta protein.

Rat Plasminogen protein (active). GTX00354-pro

GTX00354-pro Image

SDS-PAGE analysis of GTX00354-pro Rat Plasminogen protein (active).

Rat Plasminogen protein (active). GTX00354-pro

GTX00354-pro Image

WB analysis of GTX00354-pro Rat Plasminogen protein (active).

REFERENCE
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