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Rat SFTPC protein (active)

Rat SFTPC protein (active). GTX00370-pro
Rat SFTPC protein (active). GTX00370-pro
Rat SFTPC protein (active). GTX00370-pro

Cat No. GTX00370-pro

Application Functional Assay
Species Rat
Package
10 μg ($319)
APPLICATION

Application Note

Surfactant Associated Protein C (SPC) is one of the pulmonary surfactant proteins. It is a membrane protein which manufactures surfactant. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the latter can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. Besides, Monokine Induced By Interferon Gamma (MIg) has been identified as an interactor of SPC, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SPC and recombinant rat MIg. Briefly, SPC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MIg-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SPC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SPC and MIg was in a dose dependent manner.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Phe95~Ile194 (NP_059038.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

surfactant protein C,SP-C

Background

promotes reduction of surface tension in the lung [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat SFTPC protein (active). GTX00370-pro

GTX00370-pro Functional Assay Image

Functional ELISA analysis of GTX00370-pro Rat SFTPC protein (active) which can bind immobilized MIg protein.

Rat SFTPC protein (active). GTX00370-pro

GTX00370-pro Image

WB analysis of GTX00370-pro Rat SFTPC protein (active).

Rat SFTPC protein (active). GTX00370-pro

GTX00370-pro Image

SDS-PAGE analysis of GTX00370-pro Rat SFTPC protein (active).

REFERENCE
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