GeneTex
  • Country / Location Selection

United States (US)

Rat SIRT3 protein (active)

Rat SIRT3 protein (active). GTX00061-pro
Rat SIRT3 protein (active). GTX00061-pro
Rat SIRT3 protein (active). GTX00061-pro

Cat No. GTX00061-pro

Application Functional Assay
Species Rat
Package
10 μg ($309)
APPLICATION

Application Note

Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SIRT3 and recombinant rat IDH2. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IDH2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and IDH2 was in a dose dependent manner.

Observed MW

22 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro127~Glu303

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Rat SIRT3 protein (active). GTX00061-pro

GTX00061-pro Functional Assay Image

Functional ELISA analysis of GTX00061-pro Rat SIRT3 protein (active) which can bind immobilized IDH2 protein.

Rat SIRT3 protein (active). GTX00061-pro

GTX00061-pro Image

SDS-PAGE analysis of GTX00061-pro Rat SIRT3 protein (active).

Rat SIRT3 protein (active). GTX00061-pro

GTX00061-pro Image

WB analysis of GTX00061-pro Rat SIRT3 protein (active).

REFERENCE
There are currently no references for Rat SIRT3 protein (active) (GTX00061-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Rat SIRT3 protein (active) (GTX00061-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 309